Interference of soluble TNF-a receptors in immunological detection of tumor necrosis factor-a
نویسنده
چکیده
We have studied the interference of soluble tumor necrosis factor-a receptor p55 (sTNF-R55) on the quantification of tumor necrosis factor-a (TNF-a) in three immunoassays: the commercially available enzyme amplified-sensitivity immunoassay (EASLA) of Medgenix and the ELISA of Boehringer Mannheim, and an assay based on electrochemiluminescence (ECL) technology. TNF-a measurements by EASLA and ELISA were not affected by the presence of sTNF-R55, but results by the ECL method were clearly influenced by the receptor. We then performed a second set of experiments with the ECL system to examine what factors might influence the importance of interference from sTNF-R55 and soluble TNF-a receptor p75 (sTNF-R75), which influences TNF-a measurements the same as sTNFR55. We found that extending the incubation time and increasing the concentration of capture antibody decreased the interfering capacity of sTNF-R55 and sTNF-R75. A clear insight into these phenomena is of utmost importance for correct interpretation of cytokine quantification.
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